Ducolege collagen was extracted from fish skin by non-enzyme technology “Extre2Cold”, it contains highly purified, structurally intact type I collagen. It shown (1) to promote the growth and migration of fibroblasts and keratinocytes, (2) to promote fibroblasts to express type III collagen, (3) to promote fibroblasts to express hyaluronan synthases, (4) to promote keratinocyte to express moisturizing related proteins, (5) to increase skin elasticity and moisture, (6) to speed up wound healing.