This technology can harvest about 4,666 therapeutic doses from 1 g of adipose tissue cultured with our medium for 21 days, while only about 0.4 therapeutic doses can be harvested using serum-containing medium for 36 days, that is, the yield of this technology is higher than that of the serum-containing mesenchymal stem cell culture system about 11,200-fold, 15-day shorter culture time, and better stem cell characterization.