The library quality is crucial for the data quality produced by high-throughput NGS, and underestimating or overestimating the library quality can seriously affect the effectiveness of sequencing. At present, the most commonly used quantitative reagent is a fluorescent dye with Picogreen as the main component. It can only emit fluorescence when it binds to DNA double strands, and the generated fluorescence is proportional to DNA concentration within a certain range, thus playing a precise quantitative role in DNA samples.

The most common instruments used with such reagents are fluorometers and fluorescence microplate readers. Common fluorometers include Thermo Qubit4 and Qubit Flex, as well as Medclub's own products Fluo-200 and Fluo-800. However, the throughput of fluorometers is limited, and they can only reach up to 8 channels. To perform 8-channel sample detection simultaneously, standard calibration for 8 channels is also required, which is a waste of reagents. When using a fluorescence microplate reader as a detection tool, although the detection throughput has increased, there is a problem of establishing a standard curve. Due to the significant influence of the environment on fluorescent dyes, a new standard curve needs to be established for each sample detection. If the curve performance is not ideal, a new standard curve needs to be prepared to increase the experimental burden.

Feyond-F100 fluorescence microplate reader can not only perform common fluorescence experiments such as cell activity, GFP, RFP, ORAC, Ca2+flow analysis, etc., but also has a new quantitative detection function that combines the convenience of the fluorometer with the high-throughput of the microplate reader. There is no need to establish a standard curve, and only calibration of the standard points is required to achieve precise quantification of 96 samples, with a minimum detection limit of 0.1ng/μL (10μL sample).

Learn more here: https://www.medclub.com.tw/hot_487569.html