With the development of society, economy, and medical technology, the increased number of diseases that needs to be timely detected and cured, and increased health awareness has become indispensable topics of conversation during meals and leisure. Benefiting from the advancement of DNA sequencing technology, Next-Generation Sequencing (NGS) is increasingly and widely used in disease prevention and detection to safeguard people's health and well-being.

Currently, NGS technology has matured and is widely applied in fertility, tumor, and infection screenings. "To do a good job, one must first sharpen his/her tools." Patients' samples are precious, and the accuracy of tests is crucial. Therefore, we need stable and superior library preparation reagents to ensure the reliability of test results.

QIAGEN Beverly specializes in the enzyme development and production, supplying products used in 80% of all global NGS sequencing reactions, and with a strong reputation in the industry.  To simplify the library preparation process, QIAGEN Beverly has introduced the WGS Fragmentation Mix: The one single-tube reaction mix, offering an integrated solution for efficient library construction with superior library performance.

Features：

1. Fast library preparation: All-enzymatic 2.5-hours (total hands-on time: 30 minutes) workflow from enzymatic fragmentation to amplification
2. Streamlined workflow: Enzymatic fragmentation, end-repairing and dA-tailing in a single reaction step without additional sample clean-up before adapter ligation
3. Flexible DNA input and origins: Compatible with broad range of DNA inputs from 20pg–1ug from any types including FFPE, blood, fresh tissue and cDNA
4. Versatile applications: Whole genome sequencing; panel sequencing; single-cell RNA sequencing
5. Compatible with automation workflow

A streamlined 2.5-hours workflow:

Performance：

1. Minimal GC bias similar to mechanical shearing: WGS Fragmentation Mix shows similar coverage distribution to Covaris mechanical shearing and cover across extreme GC% spectra at 1ng-100ng DNA input, and it performs the best among similar products.

Figure 1: 3 bacterial genomes with different GC content equal molar mix were conducted library preparation based on the recommend protocol.

2. Flexibly adjustable fragment sizes: The desired fragment size can be achieved by adjusting the shearing time.

Figure 2: 1μg of input DNA were fragmented using the fragmentation mix for different times that 3 minutes, 6 minutes, 10 minutes, 12 minutes, 15 minutes, and 18 minutes. Data were generated and analyzed using an Agilent® Bioanalyzer 2100 High sensitivity Chip.

3. Superior DNA library yield:  The library yield is significantly higher than that of competitors before or after amplification.

Figure 3:  The library constructions were use by different brands of library preparation kits.  The library concentration was evaluated prior to amplification.

Figure 4: After DNA fragmentation by Covaris and library construction, samples with different input amounts were amplified using different brands of kits and compared for library yield. The amplification cycle numbers were kept consistent, and library quantification was performed using Qubit fluorescence quantification and qPCR.

4. High reproducibility & consistency: Superior reproducibility and consistency between run-to-run, genome-to-genome and lot-to-lot.

Figure 5: Very consistent fragmented DNA was generated across 4 replicates using the same input DNA and the same lot of the WGS fragmentation mix using fragmentation time 5min and 10min.

Figure 6: Very consistent fragmented DNA was generated across the 2 different lots of the WGS fragmentation mix on the same samples using fragmentation time 5min and 10min.

Figure 7: Very consistent fragmented DNA was generated across different genomes and the same lot of the WGS fragmentation mix using fragmentation time 5min and 10min.

Are you looking for these features? Feel free to contact us for more information on the product, and there will be a free trial kit waiting for you to claim!

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