Mechanical Stress Analysis of Transfected CHO Suspension Cells – MagLev versus Peristaltic Pumps

Keywords:

• Bioprocessing
• Pump Comparison
• Peristaltic Pumps
• Shear Stress
• Cell Viability

Facts
In comparison to a Levitronix® pump, peristaltic pumps generate flow through tube compression along a pump casing. Tube compression can generate shear stress, which results in reduced cell viability.

Test conditions
One Levitronix® and two peristaltic pumps’ shear stress generation were compared by observing their effect on respective cell viabilities. 3.4 liters of CHO cells were introduced and pumped in three identical closed-loop configurations. The flow rate of each pump was set to 3.4 lpm and different backpressures for a period of over 400 minutes. The cell viability was measured at selected times and compared.

Results
The cell viability with the Levitronix® pump system was consistently above 95%. The peristaltic pumps reached cell viabilities of 70% and 28%, respectively. Similar results have been recorded at four different pressure levels up to 0.5 bar.

Time dependent viability of the cell suspension pumped with 3.4L/min without back pressure

Reference Details: Mechanical Stress Analysis of Transfected CHO Suspension Cells - MagLev versus Peristaltic Pumps - Levitronix (digtest.us)

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Mechanical Stress Analysis of Transfected CHO Suspension Cells – MagLev versus 4-Piston Diaphragm Pumps

Keywords:

• Bioprocessing
• Pump Comparison
• 4-Piston Diaphragm Pump
• Shear Stress
• Cell Viability

Facts
In comparison to a Levitronix® pump, a 4-Piston Diaphragm Pump consists of multiple valves that restrict fluid motion to the desired direction. Valve interaction can lead to shear stress, which can result in a reduced cell viability.

Test conditions
Two Levitronix® and one 4-Piston Diaphragm pumps’ shear stress generation were compared by observing their effect on respective cell viabilities. A fixed volume of CHO cells was pumped in three identical closed loop configurations. The flow rate of each pump was set to 3.4 lpm and backpressures of 0.03, 0.3, and 0.6 bar, respectively. The cell viability was measured at selected times and compared.

Results
Both Levitronix® pump systems revealed a significantly lower cell stress effect on CHO cells than compared to the 4-Piston Diaphragm Pump. Even at high speeds, the cell death rate was about 30% lower in the Levitronix® pumps than in the 4-Piston Diaphragm Pump.

                      Cell viability for all three pumps at 3.4 lpm and 0.03 bar

Reference Details: Mechanical Stress Analysis of Transfected CHO Suspension Cells - MagLev versus 4-Piston Diaphragm Pumps - Levitronix (digtest.us)

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