SEC-MALS

 

Category: Pharmaceutical Equipment

Exhibitor: SYSTEMATIC INSTRUMENTS CO., LTD.

Booth No: M1025

 

Characteristic

 

SEC-MALS combines multi-angle light scattering with size-exclusion chromatography. It is a powerful technique for absolute characterization of macromolecules in terms of:

Molar mass
Size
Conformation
Conjugation ratio

In SEC-MALS, the SEC column serves to separate molecules by hydrodynamic volume; retention time is not used to determine molecular weight. After exiting the column of the HPLC or FPLC system, the molecules pass through a MALS detector and are probed by a laser beam.

The MALS signals, along with UV absorbance and/or differential refractive index (dRI) signals, are analyzed to quantify the analyte’s physical properties. In polymer analysis, a differential viscometer is often added to measure intrinsic viscosity. In protein and nanoparticle analysis, an online dynamic light scattering detector is often added to measure hydrodynamic radius.

SEC-MALS determines molecular weight from 200 g/mol to 1 billion g/mol. It can also determine molecular size – the rms radius, or radius of gyration R g – from 10 nm to 500 nm and beyond. By combining molar mass and size, it can also assess molecular conformation. And MALS detectors only require annual calibration.

 

Other Products

 

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  Multi-Shot pyrolyzer

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  Aura PTx particle analyzers

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  MP-PALS

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  DynaPro ® Plate Reader III

 

 

 

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