NanoDrop Microvolume Spectrophotometer

 

Model: NanoDrop

Category: Precision Medicine

Exhibitor: J & H TECHNOLOGY CO., LTD.

Booth No: N/A

 

Characteristic

 

NanoDrop Microvolume Spectrophotometer

＊ Fast, accurate quantitation and qualification of DNA, RNA, and protein
Quantify DNA, RNA, and protein samples in seconds with only 1–2 µL using Thermo Scientific NanoDrop Spectrophotometers. You can rely on the proven technology in NanoDrop instruments for accurate, fast assessments of sample concentration and purity. And by detecting and analyzing the whole spectrum, Thermo Scientific Acclaro Sample Intelligence software can find and correct contamination in your samples and even differentiate DNA from RNA.

＊ Advanced sample intelligence with Acclaro software
Acclaro Sample Intelligence Technology software (standard on NanoDrop One, NanoDrop OneC, and NanoDrop Eight spectrophotometers) analyzes absorbance across the whole UV-Vis spectrum, not just wavelengths specific to nucleic acids and proteins. It then applies powerful chemometric algorithms to find and correct for contamination in your samples. You get more reliable downstream assay results and avoid potential rework with this efficient breakthrough capability. When the software identifies a contaminant, it notifies users with a yellow contaminant icon next to the sample. Selecting the yellow icon displays the original sample, the contaminant, and the corrected analyte spectra. A table reports the corrected concentration, so you know what contaminant is present and whether to use the sample in downstream experiments. You can proceed with confidence in the rest of your analysis.

＊ Sample contaminant detection and identification
Acclaro Contaminant Identification uses sophisticated mathematical algorithms to identify contaminants in samples such as phenol, guanidine salts, and protein in nucleic acids. Based on the full absorbance spectrum, it quantifies the contaminant’s contribution to the total sample absorbance and calculates the true analyte concentration. Purity ratios alone cannot give an accurate picture of sample contamination. Some common contaminants like phenol absorb at 260 nm, the same as nucleic acids. As shown in the video, phenol contamination can cause other UV-Vis instruments to overestimate nucleic acid quantity, resulting in elevated PCR Ct values due to inadequate template. Acclaro software allows NanoDrop instruments to identify and correct for a broad range of contaminants.

＊ Differentiation between DNA and RNA
RNA contamination in genomic DNA preparations is a common issue in molecular biology workflows. Traditionally, UV absorbance technology cannot distinguish between DNA and RNA, since both absorb light at 260 nm. When measuring samples with traditional spectrometry, copurified RNA will artificially inflate the concentration of DNA, causing potential errors in downstream applications. Acclaro Sample Intelligence software can use full-spectrum data to tease apart these nucleic acids and calculate corrected DNA concentrations.

＊ Rapid time to results
One remarkable attribute of NanoDrop quantitation is how quickly results appear—within seconds after initiating a measurement on any NanoDrop spectrophotometer. In addition, with the NanoDrop Eight Spectrophotometer, you can quantify up to eight samples in less than 20 seconds, and a 96-well plate in under 6 minutes. In addition, both the NanoDrop One and Eight Spectrophotometers offer Auto-Blank and Auto-Measure capabilities, which can further streamline your workflow. These options make it unnecessary to manually select Blank or Measure repeatedly for large batches of samples.
• Blanking refers to measuring spectral absorbance of and adjusting sample results for the buffer in which samples are contained. With Auto-Blank on, blanking will be completed as soon as you lower the instrument arm.
• With Auto-Measure on, sample measurement will be completed as soon as you lower the instrument arm.
FDA regulatory compliance
Ensuring the integrity of scientific data is a requirement for many labs, from pharmaceutical and biotech companies to biobanks and academics working on clinical trials. Optional security tools for the NanoDrop One, OneC, and Eight microvolume spectrophotometers help your lab stay compliant with federal data regulations every step of the way.
Comply with US FDA 21 CFR Part 11
Control user account access, apply digital signatures, review audit logs as ilustrated in the figure below.

＊ Validate your systems
Installation qualification/Operational qualification (IQ/OQ) documents and services are available to confirm that your instrument is running within manufacturer’s specifications.
Network multiple instruments
Install Security Suite or SciVault software on a central computer to control privileges and view audit logs across multiple instruments in different labs.

＊ Streamline management
SciVault software integrates into the NanoDrop Eight user interface.

 

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