Live-Cell Kinetic Assays: Powerful Tools for Real-Time Observation of Cellular Biology

Exhibitor: GENELABS LIFE SCIENCE CORPORATION

Booth No: M929

Characteristic

Live-cell kinetic assays enable researchers to repeatedly measure parameters such as cell health, toxicity, apoptosis, or reporter gene activity over time in the same well without disrupting the cells. This real-time, non-destructive approach saves time and resources while providing richer, dynamic data to better understand cellular responses.

🧪 Key Application Areas
1️⃣ Cell Health and Cytotoxicity Assessment
* RealTime-Glo™ MT Cell Viability Assay
Measures metabolic reducing potential of cells to continuously monitor viability for up to 72 hours.
* CellTox™ Green Cytotoxicity Assay
Fluorescent dye binds to DNA of membrane-compromised cells, allowing real-time detection of cytotoxicity across various cell models.

2️⃣ Apoptosis and Necrosis Analysis
* RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay
Simultaneously detects phosphatidylserine exposure (apoptosis) and loss of membrane integrity (necrosis), providing comprehensive insights into cell death.

3️⃣ Reporter Gene Activity Monitoring
* Nano-Glo® Live Cell Assay System
Utilizes NanoLuc® luciferase for high-sensitivity, real-time reporter gene readouts.
* Nano-Glo® Endurazine™ and Vivazine™ Substrates
Designed for long-term and mid-term experiments respectively, offering stable or bright luminescence tailored to assay duration.

✨ Featured Products and Highlights
RealTime-Glo™ MT Cell Viability Assay: Non-lytic, ATP-independent, Long-term cell viability monitoring
CellTox™ Green Cytotoxicity Assay: Real-time toxicity detection, fluorescence， Cytotoxicity assessment
RealTime-Glo™ Annexin V Assay： Dual detection of apoptosis & necrosis， Mechanistic study of cell death
Nano-Glo® Live Cell Assay System：High sensitivity, short-term experiments， Reporter gene tracking
Nano-Glo® Endurazine™ Substrate： Stable luminescence, long-term experiments Long-term reporter gene assays
Nano-Glo® Vivazine™ Substrate：Bright luminescence, mid-term experiments， Mid-duration reporter gene monitoring

🔬 Experimental Design Tips
１. Choose the right substrate:
Select Nano-Glo® Live Cell (short-term), Vivazine™ (mid-term), or Endurazine™ (long-term) depending on your experiment duration for optimal signal output.

2. Multiplex detection:
Combine fluorescence and luminescence assays in the same well to monitor multiple cellular parameters and enhance data richness.

3. High-throughput compatibility:
All assays are suited for 96- and 384-well plates, enabling scalable, automated screening workflows.