🔬 HaloTag® Technology: An Innovative Platform for Protein Labeling and Imaging

Exhibitor: GENELABS LIFE SCIENCE CORPORATION

Booth No: M929

Characteristic

HaloTag® is a proprietary protein labeling system developed by Promega that enables covalent attachment of synthetic ligands to fusion proteins with high specificity and stability. This system supports a wide range of applications, including real-time imaging, protein purification, and interaction studies in both live and fixed cells.

🧪 Technology Principle
The HaloTag® protein is an engineered enzyme that forms a covalent bond with synthetic ligands containing a chloroalkane group. These ligands can carry various functional tags such as fluorophores or affinity handles, allowing researchers to label a single fusion protein with different ligands depending on the experimental need.

📸 Imaging Applications
1. Live-Cell and Fixed-Cell Imaging
HaloTag® enables precise, site-specific labeling of proteins in living or fixed cells using ligands with different fluorescent dyes—ideal for confocal, super-resolution, or time-lapse microscopy.
2. Long-Term Protein Tracking
When combined with Janelia Fluor® ligands, HaloTag® can be used to track proteins in complex 3D models like neurospheres, enabling detailed studies of neuronal development and protein dynamics.
3. High Signal-to-Noise Ratio
HaloTag® ligands offer exceptional brightness and photostability, ensuring high-quality images even at low protein expression levels, with minimal background fluorescence.

🧬 Broad Application Areas
Protein Purification & Analysis: HaloTag® ligands facilitate efficient affinity-based purification for downstream analysis.
Protein-Protein Interaction Studies: Enables label-free interaction mapping without reliance on antibodies.
Intracellular Localization & Trafficking: Monitor protein transport, localization, and turnover inside cells.
Super-Resolution Microscopy: Achieve single-molecule imaging resolution using high-performance fluorescent ligands.

🌟 Key Advantages
1. Highly Specific & Stable Labeling: Covalent binding ensures irreversible, durable labeling under diverse conditions.
2. Flexible Ligand Choices: A variety of ligands enable multiple experimental designs using the same fusion protein.
3. Versatile Cell Model Compatibility: Works seamlessly in 2D monolayers and 3D cultures.
4. Compatible with Other Technologies: Can be integrated with NanoLuc® luciferase for dual-labeling or multiplexed analyses.

📞 Contact Genelabs: Unlock the Potential of HaloTag®
Interested in learning how HaloTag® can empower your protein research or imaging workflow?
Contact Genelabs, the exclusive distributor in Taiwan, to get expert support and custom-tailored solutions.
HaloTag Info.- https://www.promega.com/resources/technologies/halotag/